Part:BBa_M36071:Design
K12 e.coli native Manganese Superoxide Dismutase
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 346
Illegal XhoI site found at 43
Illegal XhoI site found at 127 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593
Illegal NgoMIV site found at 542 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico. We optimized our sequence in DNA 2.0's Gene Designer by increasing GC content and minimizing repeats. We accomplished this by altering basepairs without altering amino acids.
Source
This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.
References
Durfee, Tim, et al. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Journal of bacteriology 190.7 (2008): 2597-2606.
Rui B, Shen T, Zhou H, Liu J, Chen J, et al. A systematic investigation of Escherichia coli central carbon metabolism in response to superoxide stress. BMC Syst Biol. 2010;4:122.
Keele Jr, Bernard B., J. M. McCord, and I. Fridovich. "Superoxide dismutase from Escherichia coli BA new manganese-containing enzyme." Journal of Biological Chemistry 245.22 (1970): 6176-6181.